Haemocytometer Diluting Pipettes – RBC & WBC Pipettes

Description

A Diluting Pipette is a tool used in to dispense a measurable quantity of blood.   Various pipettes are used in  hematology which includes RBC & WBC pipettes , Hemoglobin pipette etc.   Here we will understand  the differences between RBC &  WBC pipettes and their various uses.

1. What is the  other name of  RBC/WBC pipette  used to make dilutions of whole blood?
Thoma pipette

2. Describe the R-B-C Pipette
RBC pipette has red bead in the bulb and it has marking of 0.5, 1 and 101.

3. What is the role of red bead in pipette?
Helps in identification of pipette
Helps in mixing of diluting fluid and blood
To ensure dryness of pipette: Free mobility indicates a dry pipette

4. What are the other uses of RBC pipette?
It is used wherever higher dilution is required Eg; High total leukocyte count and total sperm count

5. Name the diluting fluid used for RBC count
Hayem’s fluid, Dacie’s fluid

6. What is the dilution used in RBC pipette?
1:200

7. What is the volume of blood the pipette delivers
It delievers 20ul or 0.02 ml of blood.
8. What is the composition of Hayem’s fluid
Hayem’s fluid: a. Sodium chloride -0.5 gm
b. Sodium sulfate -2.5 gm
c. Mercuric chloride -0.25 gm
d. Distilled water – 100 ml.

9. What is the composition of Dacie’s fluid:
Dacie’s fluid:
a. Trisodium citrate – 3 gm.
b. Formalin – 1 ml.
b. Distill water – 99 ml.